Endometrial Receptivity Analysis (ERA)

The ERA test helps avoid implantation failure by establishing the best day for embryo transfer.

This test is recommended if:

1. You have suffered an implantation failure.
2. You are undergoing assisted reproduction treatment.

What is the endometrium?

The interior of the uterus is lined with a tissue called endometrium, which is prepared each month for the arrival of an embryo and it’s the nest where the embryo implants and resides during gestation. Even the best embryo will fail to implant if conditions aren’t right.

Why use ERA?

When performing the embryo transfer in a personalized way, the chances of getting pregnant are increased after the assisted reproduction treatment.
72.5%* pregnancy rate using ERA in the first IVF cycle in any patient.

Many women undergoing IVF are unable to get pregnant, even after transferring good quality embryos. Although a good quality embryo is an important starting point, it is also important to transfer the embryo into a uterus that is ready to receive the embryo.

The timing of embryo transfer must coordinate with your body´s menstrual cycle, neither too early nor too late, but at just the right time. For most women, the best time to transfer an embryo is the same, but for some women, it can be different.

Volume of spermatozoa (amount)

Norm is 1.5-6 ml but if lower than 1.5 ml, reduced (oligozoospermia) or increased if larger than 8 ml (polyspermia).

Colour greyish, opalescent in colour.

Any changes can indicate malfunction of urinary-genital tract (for example, transparent – oligozoospermia or azoospermia, or retrograde ejaculation; reddish or brownish – presence of erythrocytes, including bleeding or increased permeability of the walls of ducts; muddy, heterogeneous – agglutination or aggregation, presence of micro flora. Approved microscopically)

Sperm PH level

Standard 7.2-8.0 slightly alkaline environment

Lower than 7.2 – acidic environment, possible dysfunction of the seminal vesicles.

Higher than 8.0 – highly alkaline environment, suspicions of prostate dysfunction (inflammation, especially indicated if leucocytes or chronic inflammation cells are discovered).

Liquefaction

Standard if the sperm liquefies within 1 hour. If longer or it does not liquefy at all, we can suspect possible prostate dysfunction.

Consistence

In standard dropping by drops from dropper. If mucous, viscous we can suspect possible prostate dysfunction.

Concentration

In standard above 15*10⁶/ml. If lower - oligozoospermia. If not present - azoospermia.

Total count

Concentration is multiplied by the quantity, in standard above 39 million in ejaculate. It is important to hand over entire amount of ejaculate in the container. If the total amount is smaller - oligozoospermia, the chances of in vivo (natural) fertilization is reduced.

Motility

Active A+B above 32%. If less – asthenozoospermia, in vivo fertilization chances are reduced. Motile A+B+C above 40%. If less than 90%, it can indicate necrozoospermia (for example, due to varicocele), it is necessary to perform evaluation of the vitality of spermatozoa and eosin nigrosine stain test, or HOS test.

Agglutination

Without a standard norm. If present, size of clusters should be evaluated, percentages of agglutinated spermatozoa, sometimes agglutination areas can be expressively differentiated (heads, tails, necks).

Aggregation

Without a standard norm. If present, size of clusters should be evaluated, other cells (usually, squamous epithelium), micro flora, traces of mucous, aggregation takes place with.